Can Proteomics Retire the Western Blot?

Understanding the Western Blot

Inquiries about various issues relating to Western blot (WB) testing are frequently posted to the Lyme disease discussion groups on the Internet. Among the most commonly asked questions are: What laboratory techniques are used to carry out the assay? What exactly is being measured? What is a “band”? How are the results interpreted? What are the CDC criteria for a “positive” test? Although some ...

Western blot analysis with quantum dot fluorescence technology: a sensitive and quantitative method for multiplexed proteomics

Western or immunoblotting analysis of protein expression in cells and tissues has been the major analytical tool for assessing molecular biological functions in basic cell biological research, pathology and drug discovery and development. With the rapid growth of our understanding of gene and protein expression pathways and limited supplies of tissue, there is an ever-increasing need for more s...

Analysis of protoscoleces-specific antigens from Echinococcus granulosus with proteomics combined with Western blot.

OBJECTIVE To establish and optimize the proteomic analysis of protoscoleces-specific antigens from Echinococcus granulosus. To provide a foundation for identifying specific antigens in the soluble proteins of E. granulosus protoscoleces for further research. METHODS Brood capsules were collected aseptically from fertile E. granulosus cysts from the livers of an infected patient. The fertile E...

The fallibility of HIV western blot.

Western blot profile in HIV infection.

BACKGROUND Although the overall sensitivity and specificity of the western blot (WB) test for detection of antibodies to various viral proteins is high, there has been a substantial difference in the timing of the appearance of antibody bands and their intensities during different stages of HIV infection. AIMS Mapping different band patterns of Western blot results and correlating them with s...